Hotspot

ndhC-trnV-UAC

LSC · 50018-50897 · 880 bp

Back to Rosularia

Pi 0.0826

MarkerSeek score 82.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 880
variable_sites: 71
pi: 0.0826
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AGAAATGCCCAGAAAATATCA	GTTCGAGTCCGTATAGCCCT	33.3	55.0	53.1	58.7	942-945 bp	1.0	47.7
ndhC-trnV-UAC_p2	AGAAATGCCCAGAAAATATCA	AGAAGGTCTACGGTTCGAGT	33.3	50.0	53.1	57.8	954-957 bp	1.0	47.6
ndhC-trnV-UAC_p3	AGAAATGCCCAGAAAATATCAT	AGAAGGTCTACGGTTCGAGT	31.8	50.0	53.8	57.8	954-957 bp	1.0	47.6
ndhC-trnV-UAC_p4	AGAAATGCCCAGAAAATATCA	GAAGGTCTACGGTTCGAGTC	33.3	55.0	53.1	57.5	953-956 bp	1.0	47.6
ndhC-trnV-UAC_p5	AGAAATGCCCAGAAAATATCA	AAGGTCTACGGTTCGAGTCC	33.3	55.0	53.1	58.8	952-955 bp	1.0	47.6