Hotspot

lhbA-trnG-UCC

LSC · 36272-36900 · 629 bp

Back to Rosularia

Pi 0.104
MarkerSeek score 85.00
Primer available yes
Label lhbA-trnG-UCC

Feature Metadata

label_name
lhbA-trnG-UCC
feature_type
igs
region
LSC
length_bp
629
variable_sites
59
pi
0.104
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
lhbA-trnG-UCC_p1 TGGATTAGTCTTTTTGGTGGGT CGTCTTCTCCTTGGCAAAGA 40.9 50.0 57.5 57.8 716-722 bp 1.0 69.5
lhbA-trnG-UCC_p2 AGTCTTTTTGGTGGGTATCCT CGTCTTCTCCTTGGCAAAGA 42.9 50.0 56.5 57.8 710-716 bp 1.0 69.5
lhbA-trnG-UCC_p3 AGTCTTTTTGGTGGGTATCCTT CGTCTTCTCCTTGGCAAAGA 40.9 50.0 57.3 57.8 710-716 bp 1.0 67.3
lhbA-trnG-UCC_p4 TGGATTAGTCTTTTTGGTGGGT TACCATTCGACCATATCCGC 40.9 50.0 57.5 57.3 689-695 bp 1.0 65.2
lhbA-trnG-UCC_p5 AGTCTTTTTGGTGGGTATCCT TACCATTCGACCATATCCGC 42.9 50.0 56.5 57.3 683-689 bp 1.0 65.2