Hotspot

trnT-GGU-psbD

LSC · 32381-33071 · 691 bp

Back to Rosularia

Pi 0.0821

MarkerSeek score 81.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 691
variable_sites: 54
pi: 0.0821
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	AAACTAGGCGCAAAGAACCA	55.0	45.0	58.1	58.0	1270-1284 bp	1.0	85.2
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	AACTAGGCGCAAAGAACCAA	55.0	45.0	58.1	58.0	1269-1283 bp	1.0	85.2
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	ACCAAAACTAGGCGCAAAGA	55.0	45.0	58.1	58.0	1274-1288 bp	1.0	85.2
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	CGCCTAATACACCTGCAACT	55.0	50.0	58.1	58.0	1371-1385 bp	1.0	84.8
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	CGCCTAATACACCTGCAACT	50.0	50.0	58.1	58.0	1374-1388 bp	1.0	84.6