Hotspot

petN-psbM

LSC · 28764-29794 · 1031 bp

Back to Rosularia

Pi 0.108

MarkerSeek score 77.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1031
variable_sites: 85
pi: 0.108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TTTTTCCTTCGGGTCCACAG	45.0	50.0	58.0	58.0	1780 bp	0.5	59.8
petN-psbM_p2	GGGTGTGAAAAGAGCAGGAA	TTTTTCCTTCGGGTCCACAG	50.0	50.0	58.0	58.0	2357 bp	0.5	59.7
petN-psbM_p3	TCTTGCTTGGGCTGCTTTAA	CTTCGGGTCCACAGAAACAA	45.0	50.0	58.0	58.0	1774 bp	0.5	59.6
petN-psbM_p4	AGTGGGTGTGAAAAGAGCAG	TTTTTCCTTCGGGTCCACAG	50.0	50.0	58.0	58.0	2360 bp	0.5	59.6
petN-psbM_p5	GGGTGTGAAAAGAGCAGGAA	CTTCGGGTCCACAGAAACAA	50.0	50.0	58.0	58.0	2351 bp	0.5	59.5