Hotspot

rpoB-trnC-GCA

LSC · 26659-27764 · 1106 bp

Back to Rosularia

Pi 0.0925

MarkerSeek score 80.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1106
variable_sites: 100
pi: 0.0925
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCAAATTAATCCCGCGGGTA	TTCCTGCTCTTTTCACACCC	50.0	50.0	58.0	58.0	1736-1766 bp	1.0	87.1
rpoB-trnC-GCA_p2	CGAGCCCATATCCTTGCTTT	TTCCTGCTCTTTTCACACCC	50.0	50.0	58.0	58.0	1984-2014 bp	1.0	86.5
rpoB-trnC-GCA_p3	CGAGCCCATATCCTTGCTTT	CTGCTCTTTTCACACCCACT	50.0	50.0	58.0	58.0	1981-2011 bp	1.0	86.5
rpoB-trnC-GCA_p4	CCGCCTACACAAGCAAATTG	TTCCTGCTCTTTTCACACCC	50.0	50.0	58.0	58.0	2176-2206 bp	1.0	86.1
rpoB-trnC-GCA_p5	CCGCCTACACAAGCAAATTG	CTGCTCTTTTCACACCCACT	50.0	50.0	58.0	58.0	2173-2203 bp	1.0	86.0