Hotspot

atpH-atpI

LSC · 13728-14414 · 687 bp

Back to Rosularia

Pi 0.0798

MarkerSeek score 82.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 52
pi: 0.0798
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	GAATACGGTCCTTGGGTTCC	45.0	55.0	58.1	58.0	1190-1194 bp	1.0	85.5
atpH-atpI_p2	AACGGAAGCAGCAGAAATCA	TGGGGAAGAATACGGTCCTT	45.0	50.0	58.1	58.0	1197-1201 bp	1.0	85.5
atpH-atpI_p3	CTCCCGGAAATTGGGCATTA	GAATACGGTCCTTGGGTTCC	50.0	55.0	57.9	58.0	1526 bp	0.5	59.2
atpH-atpI_p4	CTCCCGGAAATTGGGCATTA	TGGGGAAGAATACGGTCCTT	50.0	50.0	57.9	58.0	1533 bp	0.5	59.2
atpH-atpI_p5	CTCCCGGAAATTGGGCATTA	TTGGGGAAGAATACGGTCCT	50.0	50.0	57.9	58.0	1534 bp	0.5	59.2