Hotspot

rps16-trnQ-UUG

LSC · 5849-7127 · 1279 bp

Back to Rosularia

Pi 0.131
MarkerSeek score 84.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1279
variable_sites
162
pi
0.131
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1352-1631 bp 1.0 88.6
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1363-1642 bp 1.0 88.0
rps16-trnQ-UUG_p3 CCACAGCTGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 1373-1652 bp 1.0 86.3
rps16-trnQ-UUG_p4 TCCACAGCTGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 1374-1653 bp 1.0 86.3
rps16-trnQ-UUG_p5 ATCCACAGCTGATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.4 58.1 1375-1654 bp 1.0 84.6