Hotspot

trnA-UGC-trnI-GAU

IRa · 131607-131670 · 64 bp

Back to Rostellularia

Pi 0.0312

MarkerSeek score 70.50

Primer available yes

Label trnA-UGC-trnI-GAU

Feature Metadata

label_name: trnA-UGC-trnI-GAU
feature_type: igs
region: IRa
length_bp: 64
variable_sites: 2
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnA-UGC-trnI-GAU_p1	AGACATCCAACCCGTAATCG	TGATTTACTTCACGGGCGAG	50.0	50.0	57.4	58.0	181 bp	1.0	76.1
trnA-UGC-trnI-GAU_p2	ATCCAACCCGTAATCGCAAC	TGATTTACTTCACGGGCGAG	50.0	50.0	58.6	58.0	177 bp	1.0	75.9
trnA-UGC-trnI-GAU_p3	CATCCAACCCGTAATCGCAA	TGATTTACTTCACGGGCGAG	50.0	50.0	58.6	58.0	178 bp	1.0	75.9
trnA-UGC-trnI-GAU_p4	AGACATCCAACCCGTAATCG	AGGTCTCTGGTTCAAGTCCA	50.0	50.0	57.4	57.9	163 bp	1.0	75.4
trnA-UGC-trnI-GAU_p5	ATCCAACCCGTAATCGCAAC	AGGTCTCTGGTTCAAGTCCA	50.0	50.0	58.6	57.9	159 bp	1.0	75.2