Hotspot

trnI-GAU-trnA-UGC

IRb · 101447-101510 · 64 bp

Back to Rostellularia

Pi 0.0312

MarkerSeek score 70.50

Primer available yes

Label trnI-GAU-trnA-UGC

Feature Metadata

label_name: trnI-GAU-trnA-UGC
feature_type: igs
region: IRb
length_bp: 64
variable_sites: 2
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-GAU-trnA-UGC_p1	TGATTTACTTCACGGGCGAG	AGACATCCAACCCGTAATCG	50.0	50.0	58.0	57.4	181 bp	1.0	76.1
trnI-GAU-trnA-UGC_p2	TGATTTACTTCACGGGCGAG	ATCCAACCCGTAATCGCAAC	50.0	50.0	58.0	58.6	177 bp	1.0	75.9
trnI-GAU-trnA-UGC_p3	TGATTTACTTCACGGGCGAG	CATCCAACCCGTAATCGCAA	50.0	50.0	58.0	58.6	178 bp	1.0	75.9
trnI-GAU-trnA-UGC_p4	AGGTCTCTGGTTCAAGTCCA	AGACATCCAACCCGTAATCG	50.0	50.0	57.9	57.4	163 bp	1.0	75.4
trnI-GAU-trnA-UGC_p5	AGGTCTCTGGTTCAAGTCCA	ATCCAACCCGTAATCGCAAC	50.0	50.0	57.9	58.6	159 bp	1.0	75.2