Hotspot

petG-trnW-CCA

LSC · 65099-65223 · 125 bp

Back to Rostellularia

Pi 0.0455

MarkerSeek score 77.20

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 125
variable_sites: 5
pi: 0.0455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	TACAGACGGGGTGATCAGTT	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.1	58.0	193-208 bp	1.0	85.2
petG-trnW-CCA_p2	TACAGACGGGGTGATCAGTT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	215-230 bp	1.0	85.1
petG-trnW-CCA_p3	TACAGACGGGGTGATCAGTT	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	179-194 bp	1.0	84.9
petG-trnW-CCA_p4	ATACAGACGGGGTGATCAGT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	216-231 bp	1.0	84.4
petG-trnW-CCA_p5	ATACAGACGGGGTGATCAGT	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.8	58.0	194-209 bp	1.0	84.4