Hotspot

trnF-GAA-ndhJ

LSC · 47241-47811 · 571 bp

Back to Rostellularia

Pi 0.028

MarkerSeek score 59.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 571
variable_sites: 16
pi: 0.028
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AAAGTCTTTGCCCCAAGGAG	55.0	50.0	58.0	58.0	860-918 bp	1.0	81.9
trnF-GAA-ndhJ_p2	CTACATTGGGAATGGTCGGG	AAAGTCTTTGCCCCAAGGAG	55.0	50.0	58.0	58.0	877-935 bp	1.0	81.6
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	TGTATCACCTGGTGGACTGT	55.0	50.0	58.0	58.0	945-1003 bp	1.0	81.5
trnF-GAA-ndhJ_p4	TGCTACATTGGGAATGGTCG	AAAGTCTTTGCCCCAAGGAG	50.0	50.0	58.0	58.0	879-937 bp	1.0	81.5
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	AAAGATGCAGGGTCGTTTGT	55.0	45.0	58.0	58.0	1122-1180 bp	1.0	81.4