Hotspot

rpoB-trnC-GCA

LSC · 25830-27008 · 1179 bp

Back to Rostellularia

Pi 0.036

MarkerSeek score 80.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1179
variable_sites: 40
pi: 0.036
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AGTTCTTCTGTCAAACCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1345-1382 bp	1.0	83.6
rpoB-trnC-GCA_p2	GTCAAACCCCGGTCAATGAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.4	58.1	1336-1373 bp	1.0	82.6
rpoB-trnC-GCA_p3	TCAAACCCCGGTCAATGAAC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.4	58.1	1335-1372 bp	1.0	82.6
rpoB-trnC-GCA_p4	AGTTCTTCTGTCAAACCCCG	GCAGGACAGAAGAGAAGCAG	50.0	55.0	57.7	58.3	1396-1433 bp	1.0	81.9
rpoB-trnC-GCA_p5	CCCGGTCAATGAACCTACAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1329-1366 bp	1.0	81.9