Hotspot

trnR-UCU-atpA

LSC · 9260-9360 · 101 bp

Back to Rostellularia

Pi 0.0421

MarkerSeek score 74.50

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 101
variable_sites: 4
pi: 0.0421
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AAGAACTGCGTCCATTGTCT	GAGGAAGCAGAAGCCCTTTT	45.0	50.0	57.4	58.1	250-255 bp	1.0	78.1
trnR-UCU-atpA_p2	AAGAACTGCGTCCATTGTCT	AGGAAGCAGAAGCCCTTTTG	45.0	50.0	57.4	58.4	249-254 bp	1.0	75.8
trnR-UCU-atpA_p3	AAGAACTGCGTCCATTGTCT	GGAAGCAGAAGCCCTTTTGA	45.0	50.0	57.4	58.4	248-253 bp	1.0	75.8
trnR-UCU-atpA_p4	AAGAACTGCGTCCATTGTCT	TCAGGAACAAATGAACCGGT	45.0	45.0	57.4	57.4	218-223 bp	1.0	74.0
trnR-UCU-atpA_p5	AAAGAACTGCGTCCATTGTCT	GAGGAAGCAGAAGCCCTTTT	42.9	50.0	58.1	58.1	251-256 bp	1.0	73.3