Hotspot

rps16-trnQ-UUG

LSC · 5670-6390 · 721 bp

Back to Rostellularia

Pi 0.0526

MarkerSeek score 75.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 721
variable_sites: 29
pi: 0.0526
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GCTTTCTGCCACATCGTTTC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	619-782 bp	1.0	86.5
rps16-trnQ-UUG_p2	AACGGATTCTGCCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	648-811 bp	1.0	85.2
rps16-trnQ-UUG_p3	CAACGGATTCTGCCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.5	58.1	649-812 bp	1.0	82.8
rps16-trnQ-UUG_p4	GCTTTCTGCCACATCGTTTC	TTCGGAGGTTCGAATCCTTC	50.0	50.0	58.0	57.3	623-786 bp	1.0	81.7
rps16-trnQ-UUG_p5	GGATTCTGCCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.6	58.1	645-808 bp	1.0	81.6