Hotspot

psbB

LSC · 76102-77628 · 1527 bp

Back to Roscoea

Pi 0.00207

MarkerSeek score 37.60

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 9
pi: 0.00207
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	TACACTTCACGCTCCGAATG	AGAACACGGGGACTAGTTGA	50.0	50.0	58.0	58.0	2550-2573 bp	1.0	82.3
psbB_p2	TACACTTCACGCTCCGAATG	ATCCATTCGAAGAACACGGG	50.0	50.0	58.0	58.0	2560-2583 bp	1.0	82.1
psbB_p3	TACACTTCACGCTCCGAATG	TTCAACGGTTTGTGTAGCCA	50.0	45.0	58.0	58.0	2807-2830 bp	1.0	81.8
psbB_p4	TACACTTCACGCTCCGAATG	TTAGTGGTGTAGTCCCCCAG	50.0	55.0	58.0	58.1	2913-2936 bp	1.0	81.7
psbB_p5	TACACTTCACGCTCCGAATG	GGCGAAAGATACCAGAGCAA	50.0	50.0	58.0	58.0	2197-2224 bp	1.0	81.3