Hotspot

ndhA

SSC · 127850-130046 · 2197 bp

Back to Roscoea

Pi 0.00425

MarkerSeek score 62.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2197
variable_sites: 28
pi: 0.00425
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTCACCATTGGAAAC	GTCCACCCGGCTTCATAAAT	50.0	50.0	58.0	57.9	2420-2461 bp	1.0	81.8
ndhA_p2	CCCAGTCACCATTGGAAACA	GTCCACCCGGCTTCATAAAT	50.0	50.0	58.0	57.9	2419-2460 bp	1.0	81.8
ndhA_p3	ATGAACCCAGTCACCATTGG	GTCCACCCGGCTTCATAAAT	50.0	50.0	57.8	57.9	2424-2465 bp	1.0	80.1
ndhA_p4	GAACCCAGTCACCATTGGAA	GTCCACCCGGCTTCATAAAT	50.0	50.0	57.7	57.9	2422-2463 bp	1.0	79.6
ndhA_p5	AACCCAGTCACCATTGGAAA	GTCCACCCGGCTTCATAAAT	45.0	50.0	57.2	57.9	2421-2462 bp	1.0	75.8