Hotspot

rps15-ycf1

SSC · 131605-132061 · 457 bp

Back to Roscoea

Pi 0.0192

MarkerSeek score 60.80

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 457
variable_sites: 19
pi: 0.0192
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AAATAGGCCAGCATACGTCG	TTCTTTGGCCCAATTACCGA	50.0	45.0	58.1	57.4	758-796 bp	1.0	80.5
rps15-ycf1_p2	AACACCATGCATTGATGGGT	TTCTTTGGCCCAATTACCGA	45.0	45.0	58.1	57.4	1008-1046 bp	1.0	79.9
rps15-ycf1_p3	TACGACTCAGCTCCAGCATA	TTCTTTGGCCCAATTACCGA	50.0	45.0	57.9	57.4	1264-1302 bp	1.0	79.1
rps15-ycf1_p4	TATACGACTCAGCTCCAGCA	TTCTTTGGCCCAATTACCGA	50.0	45.0	57.9	57.4	1266-1304 bp	1.0	79.0
rps15-ycf1_p5	TCCGCCATAAAAGGTCCAAG	TTCTTTGGCCCAATTACCGA	50.0	45.0	57.9	57.4	1304-1342 bp	1.0	78.4