Hotspot

ndhG-ndhI

SSC · 126745-127213 · 469 bp

Back to Roscoea

Pi 0.0114

MarkerSeek score 69.20

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 469
variable_sites: 14
pi: 0.0114
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ACAACTCCCATACCCCCTAA	CCCTTGATTCAAGAACGATTACC	50.0	43.5	57.3	57.5	518-584 bp	1.0	52.7
ndhG-ndhI_p2	ACAACTCCCATACCCCCTAA	TCCCTTGATTCAAGAACGAT	50.0	40.0	57.3	54.4	519-585 bp	1.0	51.5
ndhG-ndhI_p3	ACAACTCCCATACCCCCTAA	ATCCCTTGATTCAAGAACGA	50.0	40.0	57.3	54.4	520-586 bp	1.0	51.4
ndhG-ndhI_p4	ATACAACTCCCATACCCCCT	CCCTTGATTCAAGAACGATTACC	50.0	43.5	57.1	57.5	520-586 bp	1.0	50.8
ndhG-ndhI_p5	ACAACTCCCATACCCCCTAA	TCCCTTGATTCAAGAACGATT	50.0	38.1	57.3	55.2	519-585 bp	1.0	50.3