Hotspot

trnH-GUG-rpl2

IRb · 88384-88431 · 48 bp

Back to Roscoea

Pi 0.0336

MarkerSeek score 71.70

Primer available yes

Label trnH-GUG-rpl2

Feature Metadata

label_name: trnH-GUG-rpl2
feature_type: igs
region: IRb
length_bp: 48
variable_sites: 5
pi: 0.0336
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-rpl2_p1	TGGATCAAGGCAGTGGATTG	GTTATCCTGCGCTTGGAAGA	50.0	50.0	57.9	58.0	184-192 bp	1.0	81.7
trnH-GUG-rpl2_p2	GGATCAAGGCAGTGGATTGT	GTTATCCTGCGCTTGGAAGA	50.0	50.0	57.9	58.0	183-191 bp	1.0	81.7
trnH-GUG-rpl2_p3	GTGGATCAAGGCAGTGGATT	GTTATCCTGCGCTTGGAAGA	50.0	50.0	57.9	58.0	185-193 bp	1.0	81.7
trnH-GUG-rpl2_p4	CGGATGTAGCCAAGTGGATC	GTTATCCTGCGCTTGGAAGA	55.0	50.0	58.1	58.0	198-206 bp	1.0	81.6
trnH-GUG-rpl2_p5	CGGATGTAGCCAAGTGGATC	CACAACCCCTTGGGGTTATC	55.0	55.0	58.1	58.2	212-220 bp	1.0	80.4