Hotspot

atpB-rbcL

LSC · 57863-58671 · 809 bp

Back to Roscoea

Pi 0.0051

MarkerSeek score 66.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 809
variable_sites: 12
pi: 0.0051
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GCCCTAGGTTTTGTGTGGAA	TGGTGACATAAGTCCCTCCT	50.0	50.0	58.0	57.4	891-921 bp	1.0	78.0
atpB-rbcL_p2	GCCCTAGGTTTTGTGTGGAA	GTGGTGACATAAGTCCCTCC	50.0	55.0	58.0	57.3	892-922 bp	1.0	77.6
atpB-rbcL_p3	TGGGCCAATAATTTGAGCGA	TGGTGACATAAGTCCCTCCT	45.0	50.0	57.9	57.4	914-944 bp	1.0	76.9
atpB-rbcL_p4	TACGCCCTAGGTTTTGTGTG	TGGTGACATAAGTCCCTCCT	50.0	50.0	57.8	57.4	894-924 bp	1.0	76.7
atpB-rbcL_p5	TGGGCCAATAATTTGAGCGA	GTGGTGACATAAGTCCCTCC	45.0	55.0	57.9	57.3	915-945 bp	1.0	76.5