Hotspot

ndhC-trnV-UAC

LSC · 53282-54871 · 1590 bp

Back to Roscoea

Pi 0.00697
MarkerSeek score 64.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1590
variable_sites
32
pi
0.00697
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCTAACCGGGGCTAAAACTC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1718-1792 bp 1.0 82.3
ndhC-trnV-UAC_p2 CTAACCGGGGCTAAAACTCC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1717-1791 bp 1.0 82.3
ndhC-trnV-UAC_p3 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1711-1785 bp 1.0 81.5
ndhC-trnV-UAC_p4 CCTAACCGGGGCTAAAACTC AGCCCAATGCAACAAAATGC 55.0 45.0 58.0 58.5 1800-1874 bp 1.0 79.9
ndhC-trnV-UAC_p5 CTAACCGGGGCTAAAACTCC AGCCCAATGCAACAAAATGC 55.0 45.0 58.0 58.5 1799-1873 bp 1.0 79.9