Hotspot

trnT-UGU-trnL-UAA

LSC · 48869-50002 · 1134 bp

Back to Roscoea

Pi 0.0167

MarkerSeek score 63.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1134
variable_sites: 44
pi: 0.0167
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	55.0	50.0	57.9	58.0	1150-1201 bp	1.0	85.9
trnT-UGU-trnL-UAA_p2	GAGTCGAACCGATGACCATC	ACCAATTTCGCCATATCCCC	55.0	50.0	58.2	58.0	1181-1232 bp	1.0	85.6
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	1158-1209 bp	1.0	85.4
trnT-UGU-trnL-UAA_p4	GAGTCGAACCGATGACCATC	TAGCGTCTACCAATTTCGCC	55.0	50.0	58.2	58.1	1189-1240 bp	1.0	85.1
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	1164-1215 bp	1.0	84.0