Hotspot

trnT-GGU-psbD

LSC · 33975-35260 · 1286 bp

Back to Roscoea

Pi 0.006

MarkerSeek score 63.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1286
variable_sites: 23
pi: 0.006
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AAGGGCCCTCTTAACTCAGT	CCCACAAAAACGAAACGGTC	50.0	50.0	58.0	58.2	1424-1479 bp	1.0	81.9
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	CCCACAAAAACGAAACGGTC	55.0	50.0	58.1	58.2	1406-1461 bp	1.0	81.8
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	CCCACAAAAACGAAACGGTC	50.0	50.0	58.1	58.2	1409-1464 bp	1.0	81.6
trnT-GGU-psbD_p4	AAGGGCCCTCTTAACTCAGT	AACCCACAAAAACGAAACGG	50.0	45.0	58.0	57.7	1426-1481 bp	1.0	81.0
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	AACCCACAAAAACGAAACGG	55.0	45.0	58.1	57.7	1408-1463 bp	1.0	80.9