Hotspot

rpoB-trnC-GCA

LSC · 27850-28839 · 990 bp

Back to Roscoea

Pi 0.00964

MarkerSeek score 53.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 990
variable_sites: 26
pi: 0.00964
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCCTCATTTCCATCATTCCAGA	AAAGGATTTGCAGTCCCCTG	45.5	50.0	57.8	58.1	1050-1291 bp	1.0	66.5
rpoB-trnC-GCA_p2	TCCCTCATTTCCATCATTCCAG	AAAGGATTTGCAGTCCCCTG	45.5	50.0	57.8	58.1	1051-1292 bp	1.0	66.5
rpoB-trnC-GCA_p3	TCATTTCCATCATTCCAGAGCA	AAAGGATTTGCAGTCCCCTG	40.9	50.0	57.8	58.1	1047-1288 bp	1.0	66.0
rpoB-trnC-GCA_p4	TCCCTCATTTCCATCATTCCA	AAAGGATTTGCAGTCCCCTG	42.9	50.0	56.7	58.1	1051-1292 bp	1.0	65.2
rpoB-trnC-GCA_p5	CCCTCATTTCCATCATTCCAGA	GCGACACCCAGATTTGAACT	45.5	50.0	57.8	58.5	1076-1317 bp	1.0	63.2