Hotspot

atpH-atpI

LSC · 14366-15486 · 1121 bp

Back to Roscoea

Pi 0.0321

MarkerSeek score 73.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1121
variable_sites: 78
pi: 0.0321
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCGGCAGAAATCAGTGGATT	ATTTTTGCAACCTTAGCCGC	50.0	45.0	58.9	57.9	1179-1267 bp	1.0	81.3
atpH-atpI_p2	CGGCAGAAATCAGTGGATTC	ATTTTTGCAACCTTAGCCGC	50.0	45.0	56.9	57.9	1178-1266 bp	1.0	79.5
atpH-atpI_p3	GCGGCAGAAATCAGTGGAT	ATTTTTGCAACCTTAGCCGC	52.6	45.0	58.2	57.9	1179-1267 bp	1.0	78.8
atpH-atpI_p4	AAGCGGCAGAAATCAGTGG	ATTTTTGCAACCTTAGCCGC	52.6	45.0	58.4	57.9	1181-1269 bp	1.0	77.0
atpH-atpI_p5	GAAGCGGCAGAAATCAGTGG	ATTTTTGCAACCTTAGCCGC	55.0	45.0	59.6	57.9	1182-1270 bp	1.0	76.1