Hotspot

trnS-GCU-trnG-UCC

LSC · 8801-9599 · 799 bp

Back to Roscoea

Pi 0.0147
MarkerSeek score 63.60
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
799
variable_sites
32
pi
0.0147
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.95
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA GGAGATACCCCTTCCCTTAAC 50.0 52.4 58.0 56.9 847-936 bp 1.0 69.7
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC GGAGATACCCCTTCCCTTAAC 50.0 52.4 58.0 56.9 846-935 bp 1.0 69.7
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA GGAGATACCCCTTCCCTTAACT 50.0 50.0 58.0 58.3 847-936 bp 1.0 68.2
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC GGAGATACCCCTTCCCTTAACT 50.0 50.0 58.0 58.3 846-935 bp 1.0 68.1
trnS-GCU-trnG-UCC_p5 AGCAATCCGACGCTTTAGTC GGAGATACCCCTTCCCTTAAC 50.0 52.4 58.4 56.9 849-938 bp 1.0 66.9