Hotspot

psbK-psbI

LSC · 7926-8459 · 534 bp

Back to Roscoea

Pi 0.0207

MarkerSeek score 64.80

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 534
variable_sites: 26
pi: 0.0207
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TCCTCACGTCCAGGATTACG	50.0	55.0	57.9	58.9	650-722 bp	1.0	79.4
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	TCCTCACGTCCAGGATTACG	45.0	55.0	58.3	58.9	647-719 bp	1.0	77.4
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	TCCTCACGTCCAGGATTACG	45.0	55.0	58.3	58.9	648-720 bp	1.0	77.4
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	TCCTCACGTCCAGGATTACG	45.0	55.0	58.3	58.9	649-721 bp	1.0	77.4
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	TTCCTCACGTCCAGGATTAC	50.0	50.0	57.9	56.7	651-723 bp	1.0	75.9