Hotspot

rps16-trnQ-UUG

LSC · 5949-7317 · 1369 bp

Back to Roscoea

Pi 0.0086
MarkerSeek score 59.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1369
variable_sites
32
pi
0.0086
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1456-1551 bp 1.0 84.2
rps16-trnQ-UUG_p2 GCCGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1454-1549 bp 1.0 84.2
rps16-trnQ-UUG_p3 AATCCACAGCCGATCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1462-1557 bp 1.0 84.0
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1438-1533 bp 1.0 83.3
rps16-trnQ-UUG_p5 TCGAGACAAGAGCATCCTCA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1508-1603 bp 1.0 82.9