Hotspot

ycf1

SSC · 125903-130441 · 4539 bp

Back to Rosa

Pi 0.00803
MarkerSeek score 43.60
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
4539
variable_sites
311
pi
0.00803
unique_haplotype_count
60
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TGTGGTACTAGTCTAGACCATCT TATCCCACCTTTCACAAGCC 43.5 50.0 57.3 57.5 280 bp 1.0 53.1
ycf1_p2 TGTGGTACTAGTCTAGACCATCT CCCACCTTTCACAAGCCTAT 43.5 50.0 57.3 57.5 277 bp 1.0 53.1
ycf1_p3 TGTGGTACTAGTCTAGACCATCT TCCCACCTTTCACAAGCCTA 43.5 50.0 57.3 58.6 278 bp 1.0 52.7
ycf1_p4 TGTGGTACTAGTCTAGACCA TATCCCACCTTTCACAAGCC 45.0 50.0 53.9 57.5 280 bp 1.0 50.2
ycf1_p5 TGTGGTACTAGTCTAGACCA CCCACCTTTCACAAGCCTAT 45.0 50.0 53.9 57.5 277 bp 1.0 50.2