Hotspot

ndhA

SSC · 121606-123953 · 2348 bp

Back to Rosa

Pi 0.00881
MarkerSeek score 59.90
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2348
variable_sites
114
pi
0.00881
unique_haplotype_count
55
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 TGACCATAATTCATGAACCCAGT CCGCCGGGTTTTATCAATTT 39.1 45.0 57.8 57.0 2532-2619 bp 1.0 54.7
ndhA_p2 TGACCATAATTCATGAACCCAGT GCCGGGTTTTATCAATTTGCA 39.1 42.9 57.8 58.0 2530-2617 bp 1.0 54.0
ndhA_p3 TGACCATAATTCATGAACCCAGT CCGCCGGGTTTTATCAATTTG 39.1 47.6 57.8 58.5 2532-2619 bp 1.0 50.6
ndhA_p4 TCATGAACCCAGTTACCATAGA CCGCCGGGTTTTATCAATTT 40.9 45.0 56.3 57.0 2522-2609 bp 1.0 50.3
ndhA_p5 TGACCATAATTCATGAACCCAGT TGGGAGAAGTTGATCGTTGA 39.1 45.0 57.8 56.5 2435-2522 bp 1.0 50.3