Hotspot

petA-psbJ

LSC · 63953-64914 · 962 bp

Back to Rosa

Pi 0.00735
MarkerSeek score 62.30
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
962
variable_sites
72
pi
0.00735
unique_haplotype_count
46
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC GCCGATACTACTGGAAGGAT 50.0 50.0 58.0 56.2 1181-1233 bp 0.988506 73.9
petA-psbJ_p2 CATTACGTGTCCAAGGCCTT GCCGATACTACTGGAAGGAT 50.0 50.0 58.2 56.2 1178-1230 bp 0.988506 72.8
petA-psbJ_p3 ATCCATTACGTGTCCAAGGC GCCGATACTACTGGAAGGATT 50.0 47.6 58.0 57.0 1181-1233 bp 0.988506 72.0
petA-psbJ_p4 ATCCATTACGTGTCCAAGGC CCGGATTAGGTTCATCTCTGT 50.0 47.6 58.0 56.9 1084-1136 bp 0.988506 71.7
petA-psbJ_p5 ATCCATTACGTGTCCAAGGC GCCGATACTACTGGAAGGATTC 50.0 50.0 58.0 58.1 1181-1233 bp 0.988506 71.5