Hotspot

ndhC-trnV-UAC

LSC · 52130-52737 · 608 bp

Back to Rosa

Pi 0.0106
MarkerSeek score 65.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
608
variable_sites
63
pi
0.0106
unique_haplotype_count
37
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 731-822 bp 1.0 85.4
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT GCACGTTGGGTCTTTGAAAC 50.0 50.0 57.9 58.2 795-886 bp 1.0 85.1
ndhC-trnV-UAC_p3 TTCTGGCCCTTTGCTAATCG AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.2 57.8 750-841 bp 1.0 84.5
ndhC-trnV-UAC_p4 TTCTGGCCCTTTGCTAATCG GCACGTTGGGTCTTTGAAAC 50.0 50.0 58.2 58.2 814-905 bp 1.0 84.3
ndhC-trnV-UAC_p5 CCTTTGCTAATCGGGGCTAA GCACGTTGGGTCTTTGAAAC 50.0 50.0 57.7 58.2 807-898 bp 1.0 83.5