Hotspot

ycf3-trnS-GGA

LSC · 45099-45985 · 887 bp

Back to Rosa

Pi 0.00945
MarkerSeek score 64.50
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
887
variable_sites
63
pi
0.00945
unique_haplotype_count
49
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTCTCCTGAAGTTGTCGGAA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 1057-1077 bp 1.0 73.6
ycf3-trnS-GGA_p2 TTTCTCCTGAAGTTGTCGGA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 1058-1078 bp 1.0 73.6
ycf3-trnS-GGA_p3 TTCTCCTGAAGTTGTCGGAA TTCCAATGCTACGCCTTCAA 45.0 45.0 56.4 57.8 1007-1027 bp 1.0 73.6
ycf3-trnS-GGA_p4 TTTCTCCTGAAGTTGTCGGA TTCCAATGCTACGCCTTCAA 45.0 45.0 56.4 57.8 1008-1028 bp 1.0 73.6
ycf3-trnS-GGA_p5 TTCTCCTGAAGTTGTCGGAA AGTTCCAATGCTACGCCTTC 45.0 50.0 56.4 58.3 1009-1029 bp 1.0 73.0