Hotspot

petN-psbM

LSC · 28876-30157 · 1282 bp

Back to Rosa

Pi 0.00818
MarkerSeek score 66.10
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1282
variable_sites
89
pi
0.00818
unique_haplotype_count
55
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 AGTAAATCTCGCTTGGGCTG AAAACAGTCAGCCAAGGTGA 50.0 45.0 58.0 57.6 1286-1410 bp 1.0 85.6
petN-psbM_p2 AGTAAATCTCGCTTGGGCTG AAACAGTCAGCCAAGGTGAT 50.0 45.0 58.0 57.3 1285-1409 bp 1.0 83.7
petN-psbM_p3 AGTAAATCTCGCTTGGGCTG AACAGTCAGCCAAGGTGATT 50.0 45.0 58.0 57.3 1284-1408 bp 1.0 83.7
petN-psbM_p4 ATGGGGAAGAAGTGGACTCT AAAACAGTCAGCCAAGGTGA 50.0 45.0 57.6 57.6 1226-1350 bp 1.0 83.2
petN-psbM_p5 ATGGGGAAGAAGTGGACTCT AACAGTCAGCCAAGGTGATT 50.0 45.0 57.6 57.3 1224-1348 bp 1.0 81.3