Hotspot

atpH-atpI

LSC · 13485-14412 · 928 bp

Back to Rosa

Pi 0.00626
MarkerSeek score 62.10
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
928
variable_sites
52
pi
0.00626
unique_haplotype_count
61
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT GCGAATCCATGGAAGGTCAT 45.0 50.0 58.0 58.0 813-1006 bp 1.0 87.7
atpH-atpI_p2 TACCTTGACCAACTCCAGGT GCGAATCCATGGAAGGTCAT 50.0 50.0 57.9 58.0 869-1062 bp 1.0 87.0
atpH-atpI_p3 AATAACGGAAGCGGCAGAAA GCGAATCCATGGAAGGTCAT 45.0 50.0 58.2 58.0 814-1007 bp 1.0 86.5
atpH-atpI_p4 GCCAATCCAGCAGCAATAAC GCGAATCCATGGAAGGTCAT 50.0 50.0 57.8 58.0 828-1021 bp 1.0 86.2
atpH-atpI_p5 GCAGTACCTTGACCAACTCC GCGAATCCATGGAAGGTCAT 55.0 50.0 58.2 58.0 873-1063 bp 0.965517 84.9