Hotspot

rps16-trnQ-UUG

LSC · 6388-7252 · 865 bp

Back to Rosa

Pi 0.0149
MarkerSeek score 62.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
865
variable_sites
96
pi
0.0149
unique_haplotype_count
59
primer_available
yes
alignment_reliability
0.95
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 883-938 bp 1.0 90.3
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GCTACTCGGAGGTTCGAATC 45.0 55.0 58.1 57.9 891-946 bp 1.0 89.9
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 894-949 bp 0.988506 89.3
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 887-942 bp 1.0 89.2
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC GCTACTCGGAGGTTCGAATC 50.0 55.0 58.2 57.9 902-957 bp 0.988506 88.9