Hotspot

trnH-GUG-psbA

LSC · 79-391 · 313 bp

Back to Rosa

Pi 0.0262
MarkerSeek score 56.10
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
313
variable_sites
43
pi
0.0262
unique_haplotype_count
44
primer_available
yes
alignment_reliability
0.88
markerseek_score
56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 398-476 bp 1.0 90.7
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 412-490 bp 1.0 90.6
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 411-489 bp 1.0 90.6
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 413-491 bp 1.0 90.6
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 55.0 55.0 58.1 57.9 348-426 bp 1.0 90.4