Hotspot

ycf1

SSC · 151209-155648 · 4440 bp

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Pi 0.0171

MarkerSeek score 50.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4440
variable_sites: 127
pi: 0.0171
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTCGGCCTCAAAAACACTT	AGTTTGGGGAATGGAAACCG	45.0	50.0	57.9	58.4	1202 bp	1.0	79.9
ycf1_p2	AGTCGGCCTCAAAAACACTT	GTTTGGGGAATGGAAACCGA	45.0	50.0	57.9	58.4	1201 bp	1.0	79.8
ycf1_p3	AGTCGGCCTCAAAAACACTT	TTGGGGAATGGAAACCGAAC	45.0	50.0	57.9	58.4	1199 bp	1.0	79.8
ycf1_p4	AGTCGGCCTCAAAAACACTT	TTTGGGGAATGGAAACCGAA	45.0	45.0	57.9	57.6	1200 bp	1.0	79.8
ycf1_p5	AGTCGGCCTCAAAAACACTT	CAATTTCCCGAGTGGTCTGA	45.0	50.0	57.9	57.5	1450 bp	1.0	78.6