Hotspot

rpl20-rps12

LSC · 95603-96353 · 751 bp

Back to Rorippa

Pi 0.011

MarkerSeek score 66.40

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 751
variable_sites: 13
pi: 0.011
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATATATCCGCGT	ACAGCCAATCCGAAATGTCA	50.0	45.0	58.0	57.8	804-864 bp	1.0	83.4
rpl20-rps12_p2	TTTTGTTCTACGCCTCCGAG	ACAGCCAATCCGAAATGTCA	50.0	45.0	57.9	57.8	818-878 bp	1.0	83.0
rpl20-rps12_p3	TCCGAGCTATATATCCGCGT	ACAAGACAGCCAATCCGAAA	50.0	45.0	58.0	57.7	809-869 bp	1.0	82.8
rpl20-rps12_p4	TCCGAGCTATATATCCGCGT	GAGGAACATGTACTCGGGTG	50.0	55.0	58.0	57.7	748-808 bp	1.0	82.7
rpl20-rps12_p5	TTTTGTTCTACGCCTCCGAG	ACAAGACAGCCAATCCGAAA	50.0	45.0	57.9	57.7	823-883 bp	1.0	82.5