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psbE-petL

LSC · 91020-92407 · 1388 bp

Back to Rorippa

Pi 0.0166

MarkerSeek score 61.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1388
variable_sites: 36
pi: 0.0166
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGA	45.0	45.0	57.9	56.3	1109-1539 bp	1.0	71.9
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1027-1457 bp	1.0	71.4
psbE-petL_p3	AATAACCAGCCCGCAATGAA	AGCTGCTAGTAGAAAACCGA	45.0	45.0	58.2	56.3	1110-1540 bp	1.0	71.2
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGA	55.0	45.0	58.2	56.3	1022-1452 bp	1.0	71.0
psbE-petL_p5	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGAA	45.0	42.9	57.9	57.0	1109-1539 bp	1.0	70.0