Hotspot

accD-psaI

LSC · 85180-85841 · 662 bp

Back to Rorippa

Pi 0.0166

MarkerSeek score 69.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 662
variable_sites: 18
pi: 0.0166
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGGATGCAATTGTACCACGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.8	57.9	829-846 bp	1.0	81.9
accD-psaI_p2	TGGATGCAATTGTACCACGT	TGCAATTGCCGGAAAGACTA	45.0	45.0	57.8	57.8	819-836 bp	1.0	81.3
accD-psaI_p3	TGGATGCAATTGTACCACGT	CCGGAAAGACTAGGCCTACT	45.0	55.0	57.8	58.2	811-828 bp	1.0	81.1
accD-psaI_p4	TGGATGCAATTGTACCACGT	CATTGCAATTGCCGGAAAGA	45.0	45.0	57.8	57.6	822-839 bp	1.0	79.9
accD-psaI_p5	TGGATGCAATTGTACCACGT	ATTGCAATTGCCGGAAAGAC	45.0	45.0	57.8	57.6	821-838 bp	1.0	79.9