Hotspot

rbcL-accD

LSC · 83069-83724 · 656 bp

Back to Rorippa

Pi 0.0264

MarkerSeek score 64.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 656
variable_sites: 28
pi: 0.0264
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AGCTTTGCGTCTATCTGGTG	TAGCTCGCCTCTGTATTCCA	50.0	50.0	58.0	57.9	1207-1213 bp	1.0	83.6
rbcL-accD_p2	TGCAATGCACGCTGTTATTG	TAGCTCGCCTCTGTATTCCA	45.0	50.0	58.0	57.9	1270-1276 bp	1.0	83.4
rbcL-accD_p3	AGCTTTGCGTCTATCTGGTG	TAACTAGAACGCTCACCCCA	50.0	50.0	58.0	58.1	1317-1329 bp	1.0	83.3
rbcL-accD_p4	TGCAATGCACGCTGTTATTG	TAACTAGAACGCTCACCCCA	45.0	50.0	58.0	58.1	1380-1392 bp	1.0	83.1
rbcL-accD_p5	TCATGGTATGCACTTCCGTG	TAGCTCGCCTCTGTATTCCA	50.0	50.0	58.0	57.9	1240-1243 bp	0.75	73.4