Hotspot

trnL-UAA-trnF-GAA

LSC · 73921-74623 · 703 bp

Back to Rorippa

Pi 0.0278

MarkerSeek score 67.60

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 703
variable_sites: 27
pi: 0.0278
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	534-793 bp	1.0	83.0
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	531-790 bp	1.0	79.8
trnL-UAA-trnF-GAA_p3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	523-782 bp	1.0	79.2
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGG	50.0	55.0	57.5	58.6	496-755 bp	1.0	78.9
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TCCTCTGCTCTACCAACTGA	50.0	50.0	57.5	57.4	506-765 bp	1.0	78.7