Hotspot

psbM-trnD-GUC

LSC · 55278-56305 · 1028 bp

Back to Rorippa

Pi 0.023
MarkerSeek score 63.30
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1028
variable_sites
41
pi
0.023
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAAGAGTGCAGTAGC TTCAATTGGTCAGAGCACCG 45.0 50.0 55.2 58.5 1136-1140 bp 1.0 56.9
psbM-trnD-GUC_p2 AGAATGAAGAGTGCAGTAGC GTTCAATTGGTCAGAGCACC 45.0 50.0 55.2 57.0 1137-1141 bp 1.0 52.8
psbM-trnD-GUC_p3 AGAATGAAGAGTGCAGTAGC TCAATTGGTCAGAGCACCG 45.0 52.6 55.2 57.8 1135-1139 bp 1.0 50.9
psbM-trnD-GUC_p4 AGAATGAAGAGTGCAGTAGC AGTTCAATTGGTCAGAGCACC 45.0 47.6 55.2 58.5 1138-1142 bp 1.0 48.8
psbM-trnD-GUC_p5 AGAATGAAGAGTGCAGTAGC AGCTACCCACTTGATTTCCA 45.0 45.0 55.2 56.4 1167-1171 bp 1.0 48.1