Hotspot

trnC-GCA-petN

LSC · 53915-54550 · 636 bp

Back to Rorippa

Pi 0.0236

MarkerSeek score 66.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 636
variable_sites: 24
pi: 0.0236
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	738-748 bp	1.0	82.7
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	739-749 bp	1.0	82.1
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CTTCCCCACACTACGAGAGA	50.0	55.0	58.1	58.2	734-744 bp	1.0	81.5
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TCTTCCCCACACTACGAGAG	50.0	55.0	58.1	58.2	735-745 bp	1.0	81.5
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	TTCCCCACACTACGAGAGAG	50.0	55.0	58.1	58.2	733-743 bp	1.0	81.5