Hotspot

trnS-GCU-trnT-CGU

LSC · 34382-35008 · 627 bp

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Pi 0.0145
MarkerSeek score 67.30
Primer available yes
Label trnS-GCU-trnT-CGU

Feature Metadata

label_name
trnS-GCU-trnT-CGU
feature_type
igs
region
LSC
length_bp
627
variable_sites
16
pi
0.0145
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnT-CGU_p1 CCAACGCTTTAGTCCACTCA GGAACGAATCACACTTTTACCA 50.0 40.9 57.8 56.9 695-736 bp 1.0 58.1
trnS-GCU-trnT-CGU_p2 AACGCTTTAGTCCACTCAGC GGAACGAATCACACTTTTACCA 50.0 40.9 58.2 56.9 693-734 bp 1.0 57.8
trnS-GCU-trnT-CGU_p3 CCAACGCTTTAGTCCACTCA AGGGATTAAAGGAACGAATCACA 50.0 39.1 57.8 57.8 705-746 bp 1.0 57.3
trnS-GCU-trnT-CGU_p4 AACGCTTTAGTCCACTCAGC AGGGATTAAAGGAACGAATCACA 50.0 39.1 58.2 57.8 703-744 bp 1.0 57.0
trnS-GCU-trnT-CGU_p5 CCAACGCTTTAGTCCACTCA GGAACGAATCACACTTTTACCAC 50.0 43.5 57.8 58.2 695-736 bp 1.0 57.0