Hotspot

trnK-UUU-rps16

LSC · 30789-31488 · 700 bp

Back to Rorippa

Pi 0.0252
MarkerSeek score 67.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
700
variable_sites
30
pi
0.0252
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAGGCAGGGATTTTTACGGA 50.0 45.0 58.2 57.1 785-800 bp 1.0 76.0
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT AGGCAGGGATTTTTACGGAA 50.0 45.0 58.2 57.1 784-799 bp 1.0 76.0
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT AAGGCAGGGATTTTTACGGA 50.0 45.0 58.2 57.1 784-799 bp 1.0 76.0
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AGGCAGGGATTTTTACGGAA 50.0 45.0 58.2 57.1 783-798 bp 1.0 76.0
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AAGGCAGGGATTTTTACGGAA 50.0 42.9 58.2 57.8 784-799 bp 1.0 73.8