Hotspot

ycf1

SSC · 107440-112476 · 5037 bp

Back to Roldana

Pi 0.00637

MarkerSeek score 56.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5037
variable_sites: 32
pi: 0.00637
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATCCTTTTGGTCCTCCTCGA	CTGCCTGTGAATAATTTGTCCA	50.0	40.9	58.0	56.9	327 bp	1.0	57.4
ycf1_p2	ATCCTTTTGGTCCTCCTCGA	TGCCTGTGAATAATTTGTCCAAC	50.0	39.1	58.0	57.9	326 bp	1.0	56.8
ycf1_p3	ATCCTTTTGGTCCTCCTCGA	TTTCTTCTTCTGCCTGTGAA	50.0	40.0	58.0	54.8	336 bp	1.0	56.2
ycf1_p4	ATCCTTTTGGTCCTCCTCGA	TGCCTGTGAATAATTTGTCCA	50.0	38.1	58.0	55.8	326 bp	1.0	55.9
ycf1_p5	TGGTCCTCCTCGAAAAACAC	CTGCCTGTGAATAATTTGTCCA	50.0	40.9	57.8	56.9	320 bp	1.0	55.8