Hotspot

trnK-TTT

LSC · 1776-1810 · 35 bp

Back to Roldana

Pi 0.0571

MarkerSeek score 69.00

Primer available yes

Label trnK-TTT

Feature Metadata

label_name: trnK-TTT
feature_type: tRNA
region: LSC
length_bp: 35
variable_sites: 2
pi: 0.0571
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-TTT_p1	GACAATGGGTTGCACAATGG	GAAAGCCGTGTGCAATGAAA	50.0	45.0	57.9	57.9	138 bp	1.0	79.4
trnK-TTT_p2	ACAATGGGTTGCACAATGGA	GAAAGCCGTGTGCAATGAAA	45.0	45.0	58.3	57.9	137 bp	1.0	77.8
trnK-TTT_p3	GACAATGGGTTGCACAATGG	GGCTTGGGGAGGGATTTTTA	50.0	50.0	57.9	57.5	107 bp	1.0	76.5
trnK-TTT_p4	GACAATGGGTTGCACAATGG	ATGAAAAATGCAAGCACGGC	50.0	45.0	57.9	58.6	124 bp	1.0	76.1
trnK-TTT_p5	GACAATGGGTTGCACAATGG	AAGCCGTGTGCAATGAAAAA	50.0	40.0	57.9	57.4	136 bp	1.0	75.8