Hotspot

rps4-trnT-TGT

LSC · 45827-46167 · 341 bp

Back to Roldana

Pi 0.0296

MarkerSeek score 58.40

Primer available yes

Label rps4-trnT-TGT

Feature Metadata

label_name: rps4-trnT-TGT
feature_type: igs
region: LSC
length_bp: 341
variable_sites: 9
pi: 0.0296
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-TGT_p1	CCTCGCCGCAATAGTATCTC	CCACTCCTTTCCCCCAAAAA	55.0	50.0	58.0	57.9	1089-1110 bp	1.0	82.7
rps4-trnT-TGT_p2	CCTCGCCGCAATAGTATCTC	CGCCATATCCCCCTTTTTGA	55.0	50.0	58.0	57.9	1370-1391 bp	1.0	82.2
rps4-trnT-TGT_p3	CCTCGCCGCAATAGTATCTC	TCGCCATATCCCCCTTTTTG	55.0	50.0	58.0	57.9	1371-1392 bp	1.0	82.2
rps4-trnT-TGT_p4	TTCGACGAAAGGGAGCAAAA	CGCCATATCCCCCTTTTTGA	45.0	50.0	58.0	57.9	1625-1637 bp	1.0	81.6
rps4-trnT-TGT_p5	TTCGACGAAAGGGAGCAAAA	TCGCCATATCCCCCTTTTTG	45.0	50.0	58.0	57.9	1626-1638 bp	1.0	81.6